Structural determinants of co-translational protein complex assembly

Protein assembly into functional complexes is critical to life s processes. While complex assembly is classically described as occurring between fully synthesized proteins, recent work showed that co-translational assembly is prevalent in human cells. However, the biological basis for the existence of this process and the identity of protein pairs that assemble co translationally remain unknown. We show that co-translational assembly is governed by structural characteristics of complexes and involves mutually stabilized subunits. Accordingly, co-translationally assembling subunits are unstable in isolation and exhibit synchronized proteostasis with their partner. By leveraging structural signatures and AlphaFold2 based predictions, we accurately predicted co-translational assembly, including pairs identities, at proteome scale and across species. We validated our predictions by ribosome profiling, stoichiometry perturbations, and smFISH experiments that revealed co-localized mRNAs. This work establishes a fundamental connection between protein structure and the translation process, highlighting the overarching impact of three dimensional structure on gene expression, mRNA localization, and proteostasis.