Whole Genome Bisulfite Sequencing of Female Zebrafish Embryos Treated with Atrazine

We exposed zebrafish embryos to 0.3, 3, and 30 ppb (µg/L) of ATZ for 72 hours post fertilization. We performed whole-genome bisulfite sequencing (WGBS) to assess the effects of developmental ATZ exposure on DNA methylation in female fish brains Next generation sequencing was performed using NovaSeq (Illumina, US) at Purdue Genomics Core Facility. All sequencing was performed at a sequencing depth of 30x coverage on the 1.5 billion base zebrafish genome