Effect of 1,25D and androgen on the junctional transfer of fluorescent tracers in LNCaP-32 cells.

LNCaP-32 cells, seeded in 6 cm dishes in replicate, were grown to 65–70% confluence. Junctional transfer was measured after microinjecting fluorescent tracers (see Materials and Methods). aThe number of fluorescent cell neighbors (Mean ± SE) 1 min (Lucifer Yellow), 3 min (Alexa-488) and 15 min (Alexa-594) after microinjection into test cell. The total number of injection trials is shown in parentheses. bCells were treated for 48 h with 1-25D, DHT (10 nM) and MB (2.5 nM). cP≤0.0001 for normal serum versus stripped and treated cells. A two tailed Student's t test was used to calculate P value assuming unequal variance. Effect of 1,25D and androgen on the junctional transfer of fluorescent tracers in LNCaP-32 cells.