Simple and Accurate Transcriptional Start Site Identification Using Smar2C2

We present Smart-Seq2 Rolling Circle to Concatemeric Consensus (Smar2C2) for the identification and quantification of transcription start sites. Smar2C2 allows for the identification of upwards of 70 million unique transcription start sites from a single sample with as little as 40 pg of RNA input. Overall design: Examination of transcriptoinal start sites using a novel technique across several plant species