Single cell RNA sequencing of nc886, a non-coding RNA transcribed by RNA polymerase III, with a primer spike-in strategy

The primary goal of this study is to develop and apply a new protocol for single cell RNA sequencing (scRNA-seq) that includes the profiling of non-coding RNAs (ncRNAs) transcribed by RNA polymerase III (Pol III), which are typically excluded in most current scRNA-seq methods.