Analysis of miRNA expression patterns in tetracycline (Tet) inducible Scl/tTA-BCR/ABL (Scl-BA) transgenic mouse hematopoietic stem-progenitor cells.. Analysis of miRNA expression patterns in tetracycline (Tet) inducible Scl/tTA-BCR/ABL (Scl-BA) transgenic mouse hematopoietic stem-progenitor cells.

To further define the roles of miRNAs in the pathogenesis of CML, we have employed miRNA microarray expression profiling as a discovery platform to identify miRNAs with the potential to drive or accelerate CML progression. Hematopoietic stem-progenitor cells miRNA profiles of BA mice after BCR/ABL expression induced for 0 week and 3 weeks were generated by miRNA microarray. Expression of six miRNAs (miR-126a-3p, miR-130a-3p,miR-142a-5p, miR-142a-3p, miR-29c-3p and miR-30c-5p) from this signature was quantified in the same RNA samples by real-time PCR, confirming low variability in BCR/ABL expressing cells. Overall design: Hematopoietic stem-progenitor cells (lin-c-kit+sca1+, LSK) of BA mice after BCR/ABL expression induced for 0 week and 3 weeks were sorted by FACS, LSK cells from three mice were mixed in each group.