Long-chain fatty acid ß-oxidation regulates embryonic development by H3K18 acetylation in mice [RNA-seq]

Fatty acids are not only important as energy sources, but also playing crucial roles in maintaining cellular homeostasis.Inhibition of long-chain fatty acid ß-oxidation (LCFAO) results in preimplantation developmental arrest, downregulated expressions of S phase-related genes, and loss of H3K18ac modification. By profiling the landscape of H3K18ac, we show that H3K18ac is enriched on the promoter regions of S phase-related genes and correlates with their expression. Taken together, we demonstrate that LCFAO could regulate mouse preimplantation development by H3K18ac, providing new evidence for metabolic-epigenetic crosstalk in regulating embryonic development. Overall design: RNA-seq analysis of mouse 8-cell and morula embryos in both control and TMZ inhibitor-treated groups.