Substrate specificity analysis of Δ9 desaturases (fD9desA, fD9desB, fD9desC, and fD9desD) from Fistulifera sp. on the basis of expression in the yeast Δole1 mutant (n = 3).

The yeast strain INVSc-1 transformed with the control vector pYES2.1/V5-His/lacZ (Control vector) served as the positive control. The yeast Δole1 mutant transformed with pYES2.1/V5-His/lacZ served as the negative control.aThe relative amount of each fatty acid was expressed as a percentage of total fatty acids (± SD) after subtracting the amount of the supplemented fatty acid from the total.