DataSheet_1_Herring roe oil in treatment of psoriasis – influence on immune cells and cytokine network.docx

BackgroundPsoriasis is a chronic immune-mediated skin disease with systemic inflammation and comorbidities. Although the disease severity may vary over time, many patients suffer from mild to moderate disease. Often local treatment will be sufficient to control the symptoms, but they may have several side effects. ω-3 polyunsaturated fatty acids have shown promising results in clinical trials with mild-to-moderate psoriasis.MethodsWe explored the impact of phospholipid bound docosahexaenoic acid and eicosapentaenoic acid in a 3:1 ratio on immune cells and cytokine networks in peripheral blood of patients with psoriasis. We investigated the inter-relation of plasma cytokine levels and disease severity in 58 patients, and explored the status of circulating immune cell activity in 18 patients with non-severe psoriasis before and during herring roe oil supplementation. Plasma concentration of 22 cytokines was measured by Luminex technology and circulating immune cells were analyzed by multicolor flow cytometry.ResultsCCL2 levels decreased over time, and IFN-γR1 increased, possibly related to the action of ω-3 polyunsaturated fatty acids. We observed a shift from naïve to effector CD4+ T cells and decreases of CD38 expression on CD4+ and CD8+ T cells, CD56bright NK cells and CD14+CD16- classical monocytes.ConclusionsThese findings support the beneficial effect of herring roe oil supplementation.

背景：银屑病是一种慢性免疫介导的皮肤疾病，伴随全身性炎症及并发症。尽管疾病严重程度可能随时间变化，但许多患者遭受的是轻度至中度的疾病。通常局部治疗足以控制症状，但可能伴有多种副作用。ω-3多不饱和脂肪酸在轻度至中度银屑病的临床试验中表现出令人鼓舞的结果。方法：本研究探讨了以3:1比例结合磷脂的二十碳六烯酸和二十碳五烯酸对银屑病患者外周血免疫细胞和细胞因子网络的影响。我们调查了58名患者血浆细胞因子水平与疾病严重程度之间的相互关系，并探讨了18名非严重银屑病患者在鳕鱼卵油补充前后循环免疫细胞活性的状态。通过Luminex技术测量了22种细胞因子的血浆浓度，并通过多色流式细胞术分析了循环免疫细胞。结果：随着时间推移，CCL2水平下降，IFN-γR1水平升高，可能与ω-3多不饱和脂肪酸的作用有关。我们观察到从原始CD4+ T细胞向效应CD4+ T细胞的转变，以及CD4+和CD8+ T细胞、CD56bright NK细胞和CD14+CD16-经典单核细胞上的CD38表达减少。结论：这些发现支持了鳕鱼卵油补充的益处。