mechanism of antigen presentation of avian dendritic cells. Gallus gallus strain:not applicable | isolate:not applicable | breed:white feathered chicken | cultivar:not applicable

The most important ability for dendritic cells (DCs) is to present antigen, which plays an irreplaceable role in recognizing and presenting viruses. Avian influenza virus (AIV), an enveloped virus with a segmented, single-stranded, negative-sense RNA genome, can cause clinical diseases in a wide range of mammalian and avian species. Previous study demonstrated H9N2 AIV inhibited the antigen present ability of avian DCs. To better understood the underline mechanism, we evaluated the entirety microRNAs and lncRNAs changing on avian DCs stimulated by H9N2 AIV and found that 19significant altered microRNAs and 332 hugely changed lncRNAs. Considered the activating function of structure protein, we turned to check which non-structural protein of AIV might inhibit the antigen presentation of DCs. Phenotype alteration and lymphocyte proliferation test demonstrated that NS2 and M42 had the ability to inhibited the maturation of LPS stimulated DCs, while NS2 might has more power to block the antigen presenting progress of DCs. Moreover, global analysis was use to illustrate how non-structural protein NS2 hijack and inhibited the antigen presentation of avian BMDCs. Result show zero altered microRNAs in NS2 transfect avian BMDCs, compared with 2186 significant changed lncRNAs. This result hint us microRNAs biogenesis might be hijacked by NS2 protein on avian BMDCs. Further experiment demonstrated that NS2 transfect significant induced 37 significant expressed microRNAs in HEK293T cells, compared with 48 significant expressed lncRNAs.