Anti-BChE antibodies detected by immunoprecipitation.

Treated mice received AAV viral vector encoding mouse BChE mutated for enhanced cocaine hydrolysis (mCocH), or human BChE with comparable mutations (hCocH). Data represent cocaine hydrolase activity adsorbed by anti-mouse IgG and IgM antibodies coupled to a solid-phase matrix (see Methods). Bound activity is expressed in arbitrary units directly proportional to the amount of cocaine-hydrolyzing enzyme. All mice transduced with the human protein, hCocH, gave a strong signal (***, p<0.001). The 16 mice transduced with the murine equivalent, mCocH, gave no significant signal as a group, though one sample was borderline.