Chorismate mutase activity of wild-type MtCM (wt) and selected MtCM library variants.^a.

aDetails to the assay conditions and calculation of individual parameters and their standard deviations are provided in Materials and Methods. bAn asterisk indicates premature termination (the last residue is Leu88 for variants 1–6 and 2–8). cCatalytic efficiency of MtCM variant alone (in the absence of MtDS). dThe apparent activation factor was estimated as described previously [10], as the ratio of CM initial velocities of the MtCM-MtDS complex (v0 (MtDS+MtCM)), normalized by MtCM-variant and chorismate concentrations, over kcat/Km for free MtCM. eRatio of initial velocity v0 (MtDS+MtCM) as in footnote c, but measured in the presence of 100 µM Phe and 100 µM Tyr, divided by v0 (MtDS+MtCM) obtained in the absence of these feedback inhibitors. Chorismate mutase activity of wild-type MtCM (wt) and selected MtCM library variants.a.