Late recurrence-associated long non-coding RNA NR2F1 antisense 1 (NR2F1-AS1) II. Late recurrence-associated long non-coding RNA NR2F1 antisense 1 (NR2F1-AS1) II

Long non-coding RNA NR2F1 antisense RNA1 (lncRNA NR2F1-AS1) was identified as the main candidate associated to late recurrence in ER-positive breast cancer clinical samples. Gain-of-function of NR2F1-AS1 induced the expression of dormancy-inducers BMP4 and DEC2, and upregulated pluripotency markers NANOG and OCT4. Representative pathways entailed to metastatic events such as HER2/Neu, hypoxia, EMT and inflammatory-response were also found enriched. Overall design: We identified the lncRNA NR2F1 antisense RNA1 (NR2F1-AS1) as the main candidate associated to late recurrence in ER-positive breast cancer. We conducted in vitro gain-of-function of NR2F1-AS1 variant1 and variant 4 in breast cancer BT474 cell line and applied microarray-based analysis.