Expression data from mouse induced chondrogenic cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18622
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We induced chondrogenic cells from adult mouse dermal fibroblast culture by transduction of c-Myc, Klf4, and SOX9 using retroviral vectors. We selected chondrogenic cells for promoter / enhancer activities of the Col11a2 gene, and established cell lines. We analyzed global gene expression profile of four induced chondrogenic cell lines (MK-4, MK-5, MK-7, and MK-10) using microarrays. Induced chondrogenic cells (passage 6) were cultured in 60 mm dishes. After reaching confluence, total RNAs were extracted.
创建时间:
2019-02-11



