Biphasic Cell Cycle Defect Causes Impaired Neurogenesis in Down Syndrome [PAX6 ChIP-Seq]

Using integrative approach involving human and mouse iPSCs, we have unravelded the cellular and molecular mechanisms of reduced neurogenesis in Down syndome Overall design: Isogenic normal and Down syndrome iPSCs were differentiated into cortical progenitor cells (CPCs) for 24 day in vitro (DIV24). Isogenic normal CPCs and down syndrome CPCs were crosslinked and sheared chramatin was prepared. ChIP was carried out using PAX6 antibody. Purified ChIPed DNA was used for high throughput seqencing. A total of samples were that one replicate of isogenic normal and one replicates of Down syndrome were used for ChIPseq library preperation. ChIPseq libraries were used for single end high throughput sequencing.