Transcriptomes of Chlamydia trachomatis L2 with Conditional GrgA deficiency

To determine the role that GrgA plays in chlamydial physiology, we constructed a Chlamydia trachomatis mutant that we term L/cgad-peig, in which the chromosomal grgA (ctl0766 or ct504) has been disrupted by Targetron mutagenesis, and the plasmid carries an inducible grgA under the control of anhydrotetracycline (ATC). RNA-Seq analysis was performed for L2/cgad-peig grown with and without ATC. Overall design: Total RNA was prepared from the midcycle and earlylate developmental stage.