Phylogenetic relationship between Gag amino acid sequences of VLP panel members (red) and Los Alamos National Laboratory (LANL) subtype reference sequences (black).

Gag subtype reference sequences were downloaded from the LANL website http://www.hiv.lanl.gov/content/sequence/NEWALIGN/align.html and filtered for subtypes present in the VLP panel. The phylogenetic tree was constructed using the neighbour joining method (Clustal W). The scale bar indicates branch length, expressed as the number of substitutions per site.