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Raw Data of Activity of a strain of

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NIAID Data Ecosystem2026-05-10 收录
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https://figshare.com/articles/dataset/Raw_Data_of_Activity_of_i_Lactobacillus_gasseri_i_and_its_exopolysaccharides_in_inflammatory_cell_model_and_on_i_Candida_albicans_i_proliferation/30581381
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These data correspond to the raw data from the article "Activity of a strain of Lactobacillus gasseri and its exopolysaccharides in inflammatory cell model and on Candida albicans proliferation". They correspond to the original files for Figures 1, 2, 3, 4, 5, Figures 1, 2 and 3 Supplementary data from the article, which aimed to observe by confocal microscopy the co-incubation of Lactobacillus gasseri and Candida albicans, each labelled with DAPI and Concanavalin A-rhodamine, respectively; and to verify the purity of the exopolysaccharides obtained, in particular the absence of protein in the exopolysaccharide extractions by SDS-PAGE and Bradford assay, or the absence of LTA by Western blot, or the absence of nucleic acids by Nanodrop. The data include images obtained from the filamentation of Candida albicans in the presence of Lg-EPS, as well as confocal microscopy images of Candida albicans, Lactobacillus gasseri, or both microorganisms co-incubated for several hours, and the original images obtained by confocal microscopy or those derived from SDS-PAGE and Western blot analyses. The data include raw data, calculations and statistics used for the relative expression of pro-inflammatory cytokines (from Caco-2 cells) and inflammatory markers by qPCR, as well as the protein levels secreted by macrophages in the presence of L. gasseri or their EPS (Lg-EPS) by ELISA. The data also includes mRNA purity measured by Nanodrop and to assess the quality of our RNA, the RNA 6000 Pico Kit for the 2100 Bioanalyzer system was used (Agilent RNA 6000 Pico Kit 5067-1513). The data also include analysis of AhR activity induced by Lg-EPS. In parallel, MTT analyses highlight the effects of Lg-EPS on the proliferation of Caco-2 cells. Some of the results obtained on the effects of Lg-EPS on C. albicans are also presented via biofilm by Crystal Violet and by absorbance measurements of Candida growth in the presence of Lg-EPS.
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2025-11-10
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