Rates of hydrogen peroxide generation in FM mitochondria from SF and BF <i>A. aegypti</i> along the blood digestion process oxidizing pyruvate and proline.

H2O2 formation in SF and in BF three different times ABM (2 h, 24 h and 72 h) A. aegypti FM mitochondria was determined. Values were expressed as mean ± SEM of pmols H2O2 generated/min/mg protein in four different mitochondrial metabolic states using: 10 mM pyruvate + proline + 1 mM ADP (PP+ADP), followed by the addition 4 µg/mL oligomycin (Oligo) and 5 µM FCCP. To assess the maximum H2O2 generation rates, 4 µg/mL antimycin a (A) were added after FCCP. Inhibition of H2O2 formation induced by BF were expressed as percentage relative to their respective metabolic states in SF. Statistical analyses between groups were performed by using ANOVA and a posteriori Tukey's test. b, p<0.01; c, p<0.001, relative to SF.