Evaluation of apoptosis of glioma cells by TUNEL assay.

(A) Single and co-cultures of glioma cells and hUCBSC and siXIAP-transfected glioma cells were seeded in 8-well chamber slides. After various treatments, TUNEL assay was done using in situ cell death detection kit (Roche) as per the manufacturer's protocol. (B) Quantitative estimation of TUNEL cells in hUCBSC treatments. (C) Quantitative estimation of TUNEL cells in siXIAP treatments. Each experiment was repeated 3 times. The results show a significant increase in the number of apoptotic cells with siXIAP and hUCBSC treatments as compared to the control and SV treatments. Inset pictures show DAPI. SV = scrambled vector. n = 3. * Significant at p<0.05.