Genome-wide maps of TBP-associated factor (TAF, Taf1) in S.cerevisiae. Saccharomyces cerevisiae

The goals of this study are to compare Next-Generation-Sequecing (NGS)-derived genome-wide occupancy of TAF (Taf1) comparing with other basal initiation components (TBP and TFIIB), histones (H3, H4, Htz1 and H4 acetylation) and histone regulator complexes (Swr1, Bdf1) in S. cerevisiae. Overall design: Examination of H3, H4ac, TBP, Bdf1 (SRP010134), Taf1 (SRP010134), Swr1 (SRP028548/PRJNA214015), Sua7 (SRP036647/PRJNA237486) and Htz1 (SRP035413/PRJNA235191) in normal growing cells. Examination of Taf1 and TBP (Spt15) in Rpb1- and Bdf1- nuclear depleted cells. The following bedGraph files were generated using the following third-party SRA data: Bdf1.bedGraph: SRP010134/SRX114994/SRR397550 Htz1.bedGraph: SRP035413/SRX433134/SRR1118262 (GEO GSE54105/GSM1308195) Swr1.bedGraph: SRP028548/SRX332103/SRR948403 Taf1.bedGraph: SRP010134/SRX114691/SRR396796 Sua7.bedGraph: SRP036647/SRX472138/SRR1197807