Targeted RNA-seq (TempO-Seq) of human induced pluripotent stem cell (hiPSC)-derived hepatocyte like cells (HLCs) treated with Nitrofurantoin against control condition

We wanted to investigate the transcriptional effect of Nitrofurantoin, a known drug induced liver injury (DILI)-compound, on hiPSC-derived HLCs. The goal was to identify which srress response pathways and biological processes are activated or altered in HLCs following Nitrofurantoin exposure. The compound is known to cause hepatotoxicity through mechanisms of oxidative stress, mitochondrial dysfunction, but the precise cellular responses in human liver models remain not fully understood.