rich-selenium.xlsx

The present study investigated the growth of Pleurotus eryngii var. tuoliensis
mycelia in liquid culture, PDA culture and cultivation substrate with different
concentrations of sodium selenite, as well as selenium enrichment and selenium
speciation in the fruiting body. The results showed that the sensitivity of P. eryngii var. tuoliensis
mycelia to selenium was divergent under different culture modes. The biomass of
P. eryngii var. tuoliensis was significantly decreased in liquid culture medium
with selenium. A relatively low concentrations of selenium (≤ 10 mg/L) promoted
mycelia growth under plate culture. In the cultivation substrate, the mycelia grew
normally at selenium concentrations of less than 300 mg/kg and reached
the highest growth rate of 3.23 mm/d at a concentration
of 40 mg/kg. A selenium concentration of 10 mg/kg promoted fruiting of
P. eryngii var. tuoliensis;
the fruiting bodies were of good quality and had a low
malformation rate. However, a selenium concentration greater than 10 mg/kg led
to reduced yield, shorter stipes, smaller caps and a higher deformity rate. In
addition, the selenium enrichment coefficient of the fruiting bodies was 1.82-3.12,
which was far higher than that of those from the control mycelia (0.29). LC-AFS
showed that selenium in the fruiting body exists mainly in the form of
selenite, SeCys, Met-SeCys and SeMet. These findings suggest that P. eryngii var. tuoliensis could be developed as a selenium-rich mushroom product for
use as a novel dietary source of bioavailable supplemental selenium.