Raw data for Figure 5: The presence of IDO and PGE-2 inhibitors reduce the suppression of T cell proliferation by Mesoangioblasts/HIDEMs

CFSE labelled PBMCs were stimulated with anti CD3/CD28 beads as before in the presence of HIDEMs/mesoangioblasts and inhibitors of IDO and Cox-2, (1-Methyl-L-trypyophan (1MT) (0.5mM) and NS-398 (1.0 uM) respectively, or both. On day 6 cells were harvested and stained with anti-CD3 and 7AAD. Cells were gated on live CD3+ populations and analysed for CFSE dilution and the numbers of cells undergoing CFSE dilution were enumerated using counting beads. Experiments were carried out in duplicates. n=4.

CFSE 标记的 PBMC 细胞在存在 HIDEMs/中胚层祖细胞及 IDO 和 Cox-2 抑制剂（分别为 1-甲基-L-三缬氨酸（1MT）(0.5mM) 和 NS-398 (1.0 uM)，或两者并用）的条件下，按照先前方法用抗 CD3/CD28 珠子进行刺激。至第 6 天，收集细胞并使用抗 CD3 和 7AAD 进行染色。细胞在活性的 CD3+ 细胞群体上进行分选，并分析 CFSE 稀释程度以及经历 CFSE 稀释的细胞数量，使用计数珠子进行计数。实验重复进行，重复次数为 4 次。