The Impact of Reproductive Tract Biofluidmechanics on Epithelial Cell Development and Sperm Motion

This thesis explores the biofluidmechanics of the mammalian female reproductive tract, focusing on the dynamic rheological properties of the Fallopian tube and their effects on epithelial cells, sperm motility, and their interactions with epithelial surfaces. It introduces a Fallopian tube epithelial cell culture model and a novel micro-Particle Image Velocimetry platform to study these interactions. Key findings reveal that fluid viscosity and tract geometry significantly influence sperm navigation and epithelial cell development and function, enhancing our understanding of fertilisation. This interdisciplinary research provides new insights into fertility issues and potential advancements in assisted reproduction technologies.