Mutational analysis of Npm1 in engrafted human leucocytes.
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Thawed MNCs of Npm1-mutated AMLs #7–9 were transplanted into irradiated NSG recipients. Engrafted CD19+ B- and CD3+ T lymphoid and CD3- CD19- CD33+ myeloid cells were sorted (sort purity >99% for each fraction) and analyzed for presence of mutant Npm1. T- and B-lymphocytes in the bone marrow of the recipients displayed wild-type Npm1, while myeloid cells were positive for mutated Npm1.
创建时间：
2015-12-02
