20240305 Gray Figure S1 data

FIG S1 Thiocyanate does not affect the minimal inhibitory concentration of nickel for Escherichia coli. E. coli strain MG1655 was grown overnight at 37°C with shaking in MOPS minimal medium containing 0.2% glucose, then normalized to A600 = 1 and rinsed twice with sterile MOPS minimal medium containing 0.2% glucose but lacking micronutrients. The resulting cell suspensions were diluted 1:40 into MOPS minimal media containing 0.2% glucose without micronutrients, supplemented with the indicated concentrations of NiSO4 and/or NaSCN. Growth curves were performed in clear 96-well plates in a Tecan Spark plate reader, incubating at 37°C with shaking and measuring A600 at 30-minute intervals for 22 hours (n=3 experimental replicates, with 1-3 technical replicates per experiment; error bars of one standard deviation). Dashed lines indicate A600 = 0.1 and t = 18 h.