GNPS - Blood-brain barrier role in metabolite regulation via excitatory amino acid transporter 3

Blood-brain barrier role in metabolite regulation via excitatory amino acid transporter 3. The questions we would like to address are: What are the metabolomic changes in whole brain regions and blood-brain barrier compartments with and without endothelial cell-specific slc1a1 glutamate transporter? Are these differences pulled out or exacerbated by mimicking an excess extracellular glutamate state using a subseizure dose of Kainic Acid? Cortex, hypothalamus, and cerebellum were isolated as well as whole brain, enriched microvessels, and plasma fractions from slc1a1 (EAAT3) endothelial cell-specific conditional knockout mice. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 2.6 um 100 A pore size Polar C18 reversed phase UHPLC column 100 x 2.1 mm (Phenomenex, Torrance, CA). LC-MS/MS data acquired in positive mode.