Single-Cell RNA-seq analysis of cell lineages in De Novo Root Regeneration

Detached Arabidopsis leaves can regenerate adventitious roots, providing a platform to study de novo root regeneration (DNRR). We performed single-cell RNA-seq analysis and revealed that regeneration primarily originated from vascular stem-cell organizer within procambium, followed by step-by-step changes of transcriptome in cell fate transition, including gradually erasing the vascular stem-cell organizer identity and recruiting the root development program. We used the adventitious rooting assay from detached Arabidopsis leaves in this study. Single-cell RNA-seq was performed using the wounded region of detached leaves cultured on B5 medium for 4 d .