Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation embryo

We studied the transcriptomic dynamics underlying human per-implantation development of trophoblast cells. Overall design: We obtained human peri-implantation embryos through blastocyst to early post -implantation stages using an in vitro coculture system, and profiled transcriptome of individual trophoblast cells collected from these embryos. By systematic analysis methods, we revealed the genetic networks regulating peri-implantation trophoblast development.