Raw data for rASFV I/II in vivo inoculation experiment in Vietnam (July 2024)

Here, we investigated the pathogenicity of rASFV I/II from Vietnam and the associated pathobiological responses in domestic pigs experimentally inoculated with rASFV I/II. Ten healthy 7-week-old female pigs were intramuscularly inoculated with an rASFV I/II strain. Clinical signs, rectal temperatures, and samples were collected daily, and necropsies were conducted postmortem. Viral DNA was detected using qPCR, and serum samples were analysed via ELISA. All inoculated pigs succumbed to infection within 5–7 days post-inoculation (dpi), with a mean of 5.5±0.7 dpi. High fever (>40.5°C) was observed at an average onset of 2.6±0.8 dpi, and the incubation period averaged 3.0±1.1 dpi. Viral DNA was first detected in blood at 2 dpi, with viral genome copies increasing steadily. Postmortem analysis revealed severe congestive splenomegaly and haemorrhagic lymphadenopathy in all pigs. High viral DNA copy numbers were detected in all organs, particularly the liver, lymph nodes, spleen, lungs, and kidneys.