Transcriptome profiling of human esophageal epithelial cells (HEECs) following treatment with Paquinimod, Sulindac , Ramatroban and 8 Gy irradiation

Human esophageal epithelial cells (HEECs) were plated in 6-well plates and divided into five treatment groups:Untreated control (0 Gy),8 Gy irradiation alone,8 Gy + Paquinimod (24 h pre-irradiation),8 Gy + Sulindac (24 h pre-irradiation),8 Gy + Ramatroban (24 h pre-irradiation).RNA was extracted 48 hours post-irradiation for RNA sequencing to investigate:Radiation-induced transcriptional changes in HEECsThe modulating effects of Paquinimod, Sulindac and Ramatroban on radiation responseComparative analysis of drug-specific transcriptomic signatures.