RNA-seq analysis of DLD-1 cell lines expressing cDNAs of subunits on human mei-cohesin complexes

The study involves trabscriptome analysis using RNA-seq of stable transgenic DLD-1 cancer cell line, which express Tet-inducible combinations of mei-cohesin subunits. The following combinations of mei-cohesin genes were expressed in DLD-1 : STAG3+SMC1beta, STAG3+SMC1beta+REC8, and STAG3+SMC1beta+RAD21L The goal of the study was to investigate the RNA transcriptional response to the induction of mei-cohesin subunits in somatic cells using Tet-on inducible transgenes activated by an rtTA transgene and doxyciline. The DLD-1 cell line, which is derived from adult male colorectal adenocarcinoma (ATCC® CCL-221™), were first stably infected with rtTA, Tet-On-STAG3 and Tet-On-SMC1beta lentifiruses This cell line was used as an additional control along with the parent cell line DLD-1. The SMC3+SMC1b cells were also separately infected with Tet-On-REC8 and Tet-On-RAD21L lentiviruses, to generate two triple transgenic cell lines modeling the somatic expression of two major meiotic cohesin complexes: REC8 and RAD21L-based. Both the drug-selected populations of transgenic cell lines and single clones were used as biological replicates.