Cytokine levels (ng/ml) in supernatant of isolated and expanded p53-specific CD4⁺ T cell lines of healthy donors.

CD4+ T cell lines were obtained from healthy donors by isolating CD154 expressing cells after restimulation with #1–#15 or #16–#30 peptides pool and expanding with PHA. p53-specific CD4+ T cell lines (50,000 cells) and peptide-pulsed autologous mo-DCs (50,000 cells) were cocultured for 20 hours and cytokines levels in the supernatant were measured by ELISA. Numbers in parentheses indicate background cytokine production against unpulsed autologous mo-DCs. Cytokine production exceeding the limit of detection and 5 times higher than background production was considered to be significant and is shown in bold.