Hubbard Brook Experimental Forest: Soil Fungal Communities, 2021-2023

Sporocarp (fungal fruiting body) observational data and fungal eDNA data extracted from soil samples collected primarily by Farrar Ransom in the summers of 2021, 2022, and 2023. Also included: detailed site metadata, soil moisture measurements, sample processing metadata, and R code used in publication analysis. Data are still being uploaded as of January 2026. The majority of these data were collected in study plots established around 2016 by Dr. Elizabeth Studer for her dissertation work. This factorial study design consists of approximately 60 plots on two hydropedological soil types beneath four canopy tree species. The four tree species we considered were white ash (Fraxinus americana), sugar maple (Acer saccharum), American beech (Fagus grandifolia), and yellow birch (Betula alleghaniensis). Some of these plots are now part of the ongoing Ash Protection Experiment. The following data tables and other entities were used in the analysis for the publication: Unique soil fungal communities are associated with disappearing ash trees in a northern temperate hardwood forest. Site_Metadata; eDNA_Abun_Table; eDNA_Taxa_Metadata; eDNA_Extraction_Metadata; iNext_Format_eDNA; Sporocarp_Count_Table; Sporocarp_Prop_Table; Soil_Moisture_Measurements; PCR_Plate_Gel_Photos; FR_DADA2_PlusFilter_CodePub; FR_eDNA_CodePub; FR_Sporocarp_CodePub; These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.