Friend or Foe? Primary Tumor represses distant metastases' growth via exosomal miRNA-1

Recently, exosome has been treated as a key mechanism for cell-to-cell communication. We thus demonstrated the roles of exosomes for the encoding messages between primary tumors and metastases. To investigate the difference between pTDE miRNA (primary tumor-derived exosomal miRNA) and mDE miRNA (metastases-derived exosomal miRNA), we isolated exosomal miRNA from each cell lines. Next, we performed small RNA sequencing for miRNA profiling. Compared with pTDE and mDE miRNAs, miR-1 is specifically abundunt in pTDE and also shows therapeutic effects to repress the growth of metastases after primary tumor resection. Overall design: Profiling both pTDEs and mDE miRNAs, we isolated exosomal miRNAs from CHMp (primary tumor) and CHMm (metastases) with two replicates.