Functional parameters for variants expressed with the α3 subunit.

The first column shows the variant expressed. The remaining columns give the mean ± SE value for parameters, derived from (N) measurements. The values for singletons (variants occurring in a single individual) are shown below the empty row. In the functional weighting analysis these variants were grouped with the variant (or wild-type) parameter closest to the measured one (see Results); the values used are shown as bold font and in parentheses immediately below the measured parameter. Receptors containing α3 and β4(K57E) showed no response to 1 µM nicotine. For the variants, the notations give the significance of the difference to the wild-type value (one way ANOVA with Dunnett’s correction; NS P>0.05, *P