Phenotypic evolution of the CR4 tumor cells (FACS analysis).

* Early passages (# 2–4) of the fast-adherent to type I collagen CR4 cells isolated from patient tumor.** Fast-adherent cells isolated from the first passage of NOD/SCID mice tumor induced by early passage.CR4 cells.*** Late passage of CR4 cells, which were twice transplanted to NOD/SCID mice and grown under.stemness-promoting conditions (passages 11–13).Note: Data from at least three different FACS analyses were expressed as means ± SD for each marker.Initially very low cell number didn't allow more complete evaluation of the stemness/pluripotency markers.