Metabolome data of fresh and conditioned in vitro oocyte maturation media and blastocyst conditioned media

This dataset includes metabolome profiles from fresh and conditioned in vitro maturation media and blastocyst conditioned media. Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry was performed on media samples and Xcalibur (RAW) files were converted to an open source mzML format (msconvert software; ProteoWizard package). The converted files were processed using the Metabolomic Analysis and Visualization Engine (MAVEN; mzroll software, Princeton University) to complete an untargeted analysis of the liquid chromatography mass spectrometry data. The pre-processed peak data tables generated by MAVEN are provided in this dataset.

Methods
Sixty µL samples of in vitro oocyte maturation media were collected before onset and after 24 hours of oocyte maturation. Sixty µL samples of blastocyst conditioned media were collected 8 days after in vitro fertilization. Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry was performed on media samples and Xcalibur (RAW) files were converted to an open source mzML format (msconvert software; ProteoWizard package). The converted files were processed using the Metabolomic Analysis and Visualization Engine (MAVEN; mzroll software, Princeton University) to complete an untargeted analysis of the liquid chromatography mass spectrometry data. The pre-processed peak data tables generated by MAVEN are provided in this dataset.