Profiling of glycerol-induced models of muscle regeneration upon melatonin treatment for 14 days in mice

Intramuscular injection of glycerol (Gly) has been reported to lead to skeletal muscle regeneration and ectopic fat deposition. We utilized glycerol injections in the tibialis anterior muscles to investigate the effects of melatonin on skeletal muscle regeneration and intramuscular fat infiltration. We characterized genome-wide expression profiles of tibialis anterior muscles from wild-type mice injured by glycerol injection following melatonin intervention. Mice that were treated with melatonin exhibited improved muscle regeneration and reduced intramuscular fat deposition, which were associated with enhanced myogenesis, remodeled lipid metabolism and reduced immune cell infiltration. Overall design: Transcriptomic profiling was performed on RNA derived from tibialis anterior muscle 14 days post glycerol injury following melatonin intervention. Tibialis anterior muscle of 12 week old wildtype C57BL/6J male mice were injected with glycerol were collected 14 days after injection. 3 biological replicates were used for each condition.