Differential miRNA expression profiles of human vascular endothelial cells (VECs) between Type-I pro-proliferative/pro-stenotic VECs and Type-II anti-proliferative/anti-stenotic VECs [Agilent-070156]

Human VECs are categorized into two groups according to their effects on the proliferation of vascular smooth muscle cells (in vitro) and the induction of stenosis in endothelia-removed arteries after transplantation (In vivo): pro-proliferative/pro-stenotic (Type-I) virsus anti-proliferative/anti-stenotic (Type-II) VECs. Since RGS5, which is a master gene responsible for aging- and oxidative stress-dependent Type-II to Type-I conversion, is the only protein-coding gene that shows differential expression profiles between Type-I and Type-II VECs, non-coding RNAs including miRNA should be working at the downstream of RGS5 for quality control of VECs. Totally 13 samples of Type-I VECs (HRMVEC, HCAEC, HMVEC, HBVEC, aged ES(KhES1)dEC, aged ES(KhES3)dEC) and Type-II VECs (young ES(KhES1)dEC, young ES(KhES3)dECs, SeViPS(BJ)dEC_lot1, SeViPS(BJ)dEC_lot2, SeViPS(HUVEC)dEC_lot1, SeViPS(HUVEC)dEC_lot2, SeViPS(HUVEC)dEC_lot3) ( (Nishio et al., World J Transl Med 4: 88-100. DOI:10.5528/wjtm.v4.i3.101) were subjected to the analyses.