Revealing an unconventional JAK1/2-STAT3 branch in macrophage IFN? signaling [RNA-Seq]

Interferon-? (IFN?) is a key cytokine in immune activation and anti-viral responses that classically signals via JAK1/2-mediated STAT1 homodimers to drive macrophage activation. Here, we identify a non-canonical signaling component in which IFN? also activates STAT3. Our results show that IFN? activates STAT3 rapidly and directly through JAK1 and JAK2. We provide the first evidence that STAT3 can form heterodimers with STAT1 in this context and demonstrate that STAT3 is co-recruited to a subset of IFN?-induced, STAT1-bound regulatory elements. While IFN? directly activates STAT3, our results reveal that its contribution to gene regulation is limited, indicating that STAT1 easily substitutes the STAT1–STAT3 heterodimer for STAT1 homodimers when STAT3 is absent. These findings uncover STAT3 as a new unconventional player in macrophage IFN? signaling, underscoring the complex and context-dependent nature of cytokine signaling networks. Overall design: RNA-seq of STAT1 knockout (STAT1KO), STAT3 knockout (STAT3KO), and wild-type (WT) BMDMs under IFN?, IL-6, IFNy-IL6-costimulation or no stimulation