The in vitro effect of orexin A on the porcine myometrial transcriptomic profile during early-implantation period

Orexins belong to the group of metabolism regulating factors connected mainly with the nervous system. Both biologically active neuropeptides, orexin A (OXA) and orexin B (OXB) are products of the proteolytic cleavage of common 130 amino-acid precursor – prepro-orexin (PPO). In the reproductive system orexins has been found to act not only on the endocrine, but also auto- and paracrine manner. In the present study we focused on the effect of OXA treatment on the global gene expression in the porcine myometrium during the most dynamic phase of the early pregnancy – the implantation period (days 15 to 16 of gestation). We analized samples obtained from 4 individuals (n=4). Obtained from the uterine horns tissue explants were divided for two groups: controls [without treatment] and orexin A treated ones. The concentration of orexin A in the culture medium was 10 µM. In vitro tissue cultures were cultivated with treatments for 24 hours.