Parambassis ranga (Indian glassy fish) genome assembly, fParRan2.1,, alternate haplotype. fParRan2.1, alternate haplotype

This project provides the Vertebrate Genomes Project genome assembly of Parambassis ranga, common name Indian glassy fish, based on a sample provided by Byrappa Venkatesh. The assembly fParRan2.1 is based on ~101x PacBio Sequel data, ~122x coverage Illumina HiSeqX data from a 10X Genomics Chromium library generated at the Wellcome Sanger Institute as well as BioNano Saphyr DLE data generated at the Rockefeller University Vertebrate Genome Laboratory and ~108x coverage HiSeqX data from a Hi-C library prepared by Arima Genomics. An initial PacBio assembly was made using Falcon-unzip, and retained haplotigs were identified using purge_haplotigs. The primary contigs were then scaffolded using the 10X data with scaff10x, then scaffolded further with BioNano hybrid scaffolding and scaffolded further still using the Hi-C data with SALSA2. Polishing and gap-filling of both the primary scaffolds and haplotigs was performed using the PacBio reads and Arrow, followed by two rounds of Illumina polishing using the 10X data and freebayes. Finally, the assembly was manually improved using gEVAL to correct mis-joins and improve concordance with the BioNano and Hi-C data. Chromosomes are named by synteny to medaka. The assembly is provided by the Wellcome Sanger Institute and Cambridge University team (https://www.sanger.ac.uk/science/data/vertebrate-genomes-sequencing) of the Vertebrate Genomes Project (http://vertebrategenomesproject.org). The data under this project are made available subject to the Genome10K data use policies (https://genome10k.soe.ucsc.edu/data-use-policies).