Binding affinities of the ASOs for the on- and off-target sites of the SOD-1 minigene mRNA transcribed and spliced in the nuclear extract.
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Dissociation constants (Kd) were determined as described in in the Materials and Methods and figure S3. Differences in the binding affinities (ΔKd) between the two targets were calculated by dividing the Kd of the ASOs for the off-target sites by the Kd for the on-target sites.
创建时间：
2015-12-02
