Dendritic excitations govern back-propagation via a spike-rate accelerometer

These data are described in: https://doi.org/10.1038/s41467-025-55819-9. Dendritic voltage imaging was performed in mouse hippocampal brain slices. Optopatch-V (CAG::LR-Voltron2-p2a-LR-CheRiff-eYFP; Addgene #203228) was expressed via in utero electroporation. The voltage indicator was loaded with JFx608 HTL dye. Voltage imaging and targeted optogenetic stimulation were performed on a custom one-photon microscope. Please see Source Data File on the Nature Communications website for an index mapping files on DANDI to paper figure panels. To clarify the patch clamp amplifier settings used in this dataset: Daq_aof_channel_2 represents the command voltage sent from the DAQ to the amplifier, with a sensitivity of 400 pA/V. For example, 1 V in this trace corresponds to a 400 pA current injection. Daq_aif_channel_2 shows the feedback signal from the amplifier, configured at 2 nA/V, so 0.2 V also corresponds to 400 pA. These two signals should therefore indicate the same injected current. For membrane voltage traces (Daq_aif_channel_1), the amplifier gain was set to 50 mV/V. This means that a raw signal ranging from –1.5 to +1.0 V corresponds to an actual membrane potential of –75 mV to +50 mV.