CU_UROGYN_USL_DATABASE_042620.xlsx

Excel database of patient demographic information and USL tissue characteristics from control patients and patients treated for pelvic organ prolapse. Specimens were evaluated using quantitative and semi-quantitative measures by a histopathologist who was blinded to non-prolapse or prolapse diagnosis. Scoring methods/criteria were as follows.<br><br>Fibrillar collagen (COL)<br>Fibrillar collagen in USL specimens was determined by computerized image analysis where the percent of area covered by trichrome-stain positive collagen fibers was quantified using the Computer Program SlideBook v6.0 All other measurements were generated by subjective measures by the histopathologist. Photomicrographic images of all specimens used for this study are present in .PDF files separated by POP and CTRL groups. Categories, acronynms where used, and scoring criteria are listed as follows:<br>Total Non-Vascular Smooth Muscle (SM)<br>Scoring:3: No loss in muscle fascicles, no fiber dropout in fascicles2: 10-33% loss of muscle fascicles and fibers1: 33-66% loss of muscle fascicles and fibers0: 66-100% loss of muscle fascicles and<br>Smooth Muscle Fiber Drop Out (SmFDO)<br>Scoring:<br>0: less than 10% loss of fibers from any fascicle1: 10-33% loss of fibers from any fascicle2: 33-66% loss of fibers from any fascicle3: more than 66% loss of fibers from any fascicle<br>Muscle Fiber Vesicles (MUSC_VASC)<br>Scoring:0: No vesicles in any fibers1: Vesicles in 10-33% of fibers2: Vesicles in 33-66% of fibers3: Vesicles in 66-100% of fibers<br>Adipose Tissue (ADIPO)<br>Scoring:0: No Adipose within the Ligament1: 10-20% of the area covered by adipose within the ligament2: 20-35% of the area covered by adipose within the ligament3: more than 40% of the area covered by adipose within the ligament<br>Polymorphonuclear (PMN) Cell Inflammation (PMN-Inflam)<br>Scoring:0: No PMNs in the tissue, few in the vasculature1: A few PMNs in the tissue and vessels of a small area2: Abundant PMNs in a small, localized area or medium number of PMNs over the whole USL3: Abundant PMNs over the whole USL<br>Peri-Neural Inflammation (PN-Inflam)<br>Scoring:0: No PMNs within 3 cell diameters of any neural bundle1: A few PMNs within 3 cell diameters of any neural bundle2: A few PMNs near all neural bundles or higher numbers near just one neural bundle3: Abundant PMNs near any or all neural bundles<br>Neointimal Hyperplasia (NIH)<br>Scoring:: 1 layer of intima, the endothelial cell layer1: 2-4 layers of intima including the endothelial cell layer2: 5-8 layers of intima3: more than 8 layers of intima<br>(Blood) Vessel Quantity (VESSELS)<br>Scoring:0: No medium or large arteries or veins visible1: Only 1 medium size artery or multiple smaller arteries2: 1-2 large arteries and veins3: Multiple cross-sections of multiple large arteries