Replication Data for: Evaluation of the cytotoxic and antimicrobial potential of epiphytic biomass obtained from Laminaria hyperborea biorefinery side-streams

This dataset describes the calculations and the related methods used to determine the EC50 values-cytotoxicity against 5 cell lines (MOLM13, PC3, MCF7, NRK, H9C2) of the crude extracts and flash fractions of Laminaria's epiphytes. In order to assess the cytotoxicity, we performed the WST-1 cell proliferation assay, a colorimetric assay, using 96 well plates [1]. By measuring the UV absorbance, we calculated the % cell survival and then, the EC50 (concentration that reduces the viability of cells by 50%) using four-parameter regression analysis (dose response curve). We also performed one-way statistical analysis to determine differences between the extracts and between the fractions. [1] Diaz, A.E.C.; Herfindal, L.; Andersen, H.L.; Fossen, T. Cytotoxic Natural Products Isolated from Cryptogramma Crispa (L.) R. Br. Molecules 2023, 28, 7723, https://doi.org/10.3390/molecules28237723