GNPS - Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung

Lipidomics, proteomics and metabolomics characterization of the ontogeny of lipid, protein and metabolite changes during normal postnatal lung development. Samples were extracted with a chloroform methanol mixture, partitioning the samples into 3 fractions: protein, lipid and metabolite. The protein fraction was digested with trypsin, then analyzed by LC-MS/MS. The metabolite fraction was derivatized with MSTFA, then analyzed by GC-MS. The lipid fraction was analyzed by LC-MS/MS.