Supporting Dataset to accompany "Freshwater Unionid Mussels Threatened by Predation of Round Goby (Neogobius melanostomus)"

We provide supporting information to accompany the manuscript "Freshwater Unionid Mussels Threatened by Predation of Round Goby (Neogobius melanostomus)," published in Scientific Reports (see citation below). In the study, we consider the propensity of Round Goby to prey upon indigenous freshwater mussel species. First, we conducted lab experiments where Round Gobies were given the opportunity to feed on juvenile unionid mussels and macroinvertebrates, revealing rates and preferences of consumption. Here, we present information about the laboratory stream table setup and results. Second, we investigated Round Gobies collected from their newly-invaded stream habitats in the French Creek watershed, using novel DNA metabarcoding methods to reveal the specific mussel species they consumed. Here, we provide datasets on the primers used for the amplification, samples included in the sequencing analysis, and metabarcoding gene sequences of 15 indigenous unionid mussel species. Further, we provide selected photos (e.g., of indigenous unionid mussels and invasive Round Goby) from the study. The sampling of aquatic life and experiments on animal subjects were carried out in accordance with standard research protocols. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.